CREBBP Polyklonaler Antikörper

CREBBP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22277-1-PBS

Synonyme

CBP, KAT3A, RSTS

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  WB analysis using 22277-1-AP (same clone as 22277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22277-1-AP (CREBBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22277-1-AP (same clone as 22277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22277-1-AP (same clone as 22277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22277-1-AP (same clone as 22277-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREBBP antibody (22277-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 22277-1-AP (same clone as 22277-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CREBBP Polyclonal antibody (22277-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22277-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CREBBP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CREB binding protein
• Berechnetes Molekulargewicht: 265 kDa
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: NM_004380
• Gene symbol: CREBBP
• Gene ID (NCBI): 1387
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The acetyltransferase CREB-binding protein (CREBBP; also known as CBP or KAT2A) and E1A-binding protein P300 (EP300; also known as P300 or KAT2B) are two closely related regulators. Both are widely expressed within and outside the hematopoietic system and serve as tumor activators or suppressors depending on the situation. CREBBP/EP300 regulate various key physiological functions, including cell apoptosis, proliferation and differentiation, DNA repair, and somatic cell reprogramming.