CRELD1 Polyklonaler Antikörper

CRELD1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29903-1-PBS

Synonyme

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  WB analysis of rat lung using 29903-1-AP (same clone as 29903-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 29903-1-AP (same clone as 29903-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 29903-1-AP (same clone as 29903-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 29903-1-AP (same clone as 29903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 29903-1-AP (same clone as 29903-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29903-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CRELD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRELD1 fusion protein Ag31593
• Vollständiger Name: cysteine-rich with EGF-like domains 1
• Berechnetes Molekulargewicht: 45KD
• Beobachtetes Molekulargewicht: 45 kDa, 70-75 kDa
• GenBank-Zugangsnummer: NM_001031717
• Gene symbol: CRELD1
• Gene ID (NCBI): 78987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).