CREST Monoklonaler Antikörper

CREST Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D5D10

Kat-Nr. : 60314-1-Ig

Synonyme

CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1

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  WB analysis of NIH/3T3 using 60314-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60314-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60314-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 60314-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 60314-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, COLO 320-Zellen, MCF-7-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60314-1-Ig bindet in WB, ELISA CREST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CREST fusion protein Ag3119
• Vollständiger Name: synovial sarcoma translocation gene on chromosome 18-like 1
• Berechnetes Molekulargewicht: 396 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC034494
• Gene symbol: CREST
• Gene ID (NCBI): 26039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).