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CRH/CRF Polyklonaler Antikörper

CRH/CRF Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26848-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using CRH/CRF Polyclonal antibody (26848-1-AP)

    WB analysis using 26848-1-AP (same clone as 26848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26848-1-AP (CRH/CRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IHC staining of human placenta using 26848-1-AP (same clone as 26848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IHC staining of human placenta using 26848-1-AP (same clone as 26848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IF Staining of rat brain using 26848-1-AP (same clone as 26848-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody (26848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

26848-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CRH/CRF und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CRH/CRF fusion protein Ag25226
Vollständiger Name CRH/CRF
Berechnetes Molekulargewicht 21 kDa
Beobachtetes Molekulargewicht27 kDa
GenBank-ZugangsnummerBC011031
Gene symbol CRH
Gene ID (NCBI) 1392
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.