CRHR2 Polyklonaler Antikörper

CRHR2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25267-1-AP

Synonyme

Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2, CRF R 2, CRF R2, CRF2R

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  WB analysis using 25267-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 25267-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

25267-1-AP bindet in WB, IHC, IF, ELISA CRHR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: corticotropin releasing hormone receptor 2
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: NM_001883
• Gene symbol: CRHR2
• Gene ID (NCBI): 1395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRHR2 is a receptor for corticotropin-releasing factor (CRF) and belongs to the G-protein coupled receptor family. It shows high affinity for CRF and also binds to related peptides such as urocortin I, II, and III. This receptor is involved in mediating the endocrine, autonomic, and behavioral responses to stress and immune challenges.

Publikationen

Species	Application	Title
human	WB,IF	Pharmaceuticals (Basel); A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors; Authors - Yueshan Sun
mouse	WB	Cell Signal; Activation of CRHR1 contributes to cerebral endothelial barrier impairment via cPLA2 phosphorylation in experimental ischemic stroke.; Authors - Changchun Cao
rat,human	WB,IHC	Neurogastroenterol Motil; Lidocaine ameliorates intestinal barrier dysfunction in irritable bowel syndrome by modulating corticotropin-releasing hormone receptor 2; Authors - Yanrong Wang
mouse	IF	Reprod Sci; Melatonin Protects Against Mitochondrial Dyshomeostasis and Ovarian Damage Caused by Chronic Unpredictable Mild Stress Through the eIF2α-AFT4 Signaling Pathway in Mice; Authors - Si-Min Ding
rat	WB	Food Res Int; Lactobacillus reuteri or Lactobacillus rhamnosus GG intervention facilitates gut barrier function, decreases corticosterone and ameliorates social behavior in LPS-exposed offspring; Authors - Xinyuan Wang