CRIPT Polyklonaler Antikörper

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• KD/KO Validated

CRIPT Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11211-1-PBS

Synonyme

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  WB analysis of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  IP experiment of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  IP result of anti-CRIPT (IP:11211-1-AP, 4ug; Detection:11211-1-AP 1:500) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  Immunofluorescent analysis of L02 cells using 11211-1-AP (CRIPT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11211-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA CRIPT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRIPT fusion protein Ag1708
• Vollständiger Name: cysteine-rich PDZ-binding protein
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC018653
• Gene symbol: CRIPT
• Gene ID (NCBI): 9419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains