CRISP3 Polyklonaler Antikörper

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CRISP3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14847-1-PBS

Synonyme

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  WB analysis using 14847-1-AP (same clone as 14847-1-PBS)

  human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 14847-1-AP (same clone as 14847-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 14847-1-AP (same clone as 14847-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14847-1-AP (same clone as 14847-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human placenta using 14847-1-AP (same clone as 14847-1-PBS)

  IP result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14847-1-PBS bindet in WB, IHC, IP, Indirect ELISA CRISP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cysteine-rich secretory protein 3
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_006061
• Gene symbol: CRISP3
• Gene ID (NCBI): 10321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.