CDK12/CRKRS Rekombinanter Antikörper

CDK12/CRKRS Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250880E5

Kat-Nr. : 86296-3-PBS

Synonyme

CrkRS, CDC2 related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, CDC2-related protein kinase 7, CDK12

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  WB analysis using 86296-3-RR (same clone as 86296-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86296-3-RR (same clone as 86296-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86296-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86296-3-RR against Human CDK12/CRKRS were performed. The affinity constant is 0.106 nM.

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Geprüfte Anwendungen

Produktinformation

86296-3-PBS bindet in WB, IF/ICC, Indirect ELISA CDK12/CRKRS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK12/CRKRS fusion protein Ag25312
• Vollständiger Name: Cdc2-related kinase, arginine/serine-rich
• Berechnetes Molekulargewicht: 1490 aa, 164 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC150265
• Gene symbol: CDK12
• Gene ID (NCBI): 51755
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).