CRMP1 Polyklonaler Antikörper

CRMP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10317-1-AP

Synonyme

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

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Galerie der Validierungsdaten

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  WB analysis using 10317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10317-1-AP (CRMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10317-1-AP (CRMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 10317-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CRMP1 antibody (10317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 10317-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CRMP1 antibody (10317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Maushirngewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10317-1-AP bindet in WB, IHC, IF, CoIP, ELISA CRMP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRMP1 fusion protein Ag0369
• Vollständiger Name: collapsin response mediator protein 1
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC000252
• Gene symbol: CRMP1
• Gene ID (NCBI): 1400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

Publikationen

Species	Application	Title
human	WB	Chem Biol Interact; Exposure to Benzo(a)pyrene damages mitochondrial function via suppressing mitochondrial melatonin receptors in ovarian corpus luteum during early pregnancy; Authors - Jie Yang
rat	WB	Molecules; Senescent Phenotype of Astrocytes Leads to Activation of BV2 Microglia and N2a Neuronal Cells Death; Authors - Wenyou Zhang
mouse,human	WB,CoIP	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen