CRMP2 Polyklonaler Antikörper

CRMP2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14686-1-PBS

Synonyme

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  WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14686-1-AP (same clone as 14686-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 14686-1-AP (CRMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14686-1-AP (same clone as 14686-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CRMP2 antibody (14686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14686-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CRMP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRMP2 fusion protein Ag5985
• Vollständiger Name: dihydropyrimidinase-like 2
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62-66 kDa
• GenBank-Zugangsnummer: BC056408
• Gene symbol: CRMP2
• Gene ID (NCBI): 1808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).