CROP Polyklonaler Antikörper

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• KD/KO Validated

CROP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 14504-1-AP

Synonyme

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

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  WB analysis of Jurkat using 14504-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14504-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14504-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14504-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14504-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14504-1-AP

  IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IHC staining of human ovary cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14504-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, Maushirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Eierstockgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

14504-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CROP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CROP fusion protein Ag5961
• Vollständiger Name: cisplatin resistance-associated overexpressed protein
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC056409
• Gene symbol: CROP
• Gene ID (NCBI): 51747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

Publikationen

Species	Application	Title
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB	Cell Rep; Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.; Authors - Noah J Daniels ; KD Validated
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang
human	WB	Curr Issues Mol Biol; Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma; Authors - Yushan Hou
mouse	IHC	Inorg Chem; Studies of Anticancer Activities In Vitro and In Vivo for Butyltin(IV)-Iridium(III) Imidazole-Phenanthroline Complexes with Aggregation-Induced Emission Properties; Authors - Yiwei Sun
human	WB	J Biol Chem; The NEDD4-binding protein N4BP1 degrades mRNA substrates through the coding sequence independent of nonsense-mediated decay; Authors - Wen Zheng