CROP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CROP Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14504-1-AP

Synonyme

CRE associated protein 1, CREAP 1, CREAP1, CROP, Luc7 like protein 3, LUC7A, LUC7L3, O48, OA48 18

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 14504-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14504-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14504-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14504-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14504-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14504-1-AP

  IP Result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IHC staining of human pancreas cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14504-1-AP (CROP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 14504-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14504-1-AP (CROP antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 14504-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14504-1-AP (CROP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, Maushirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

14504-1-AP bindet in WB, IP, IHC, IF, ELISA CROP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CROP fusion protein Ag5961
• Vollständiger Name: cisplatin resistance-associated overexpressed protein
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC056409
• Gene symbol: CROP
• Gene ID (NCBI): 51747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

Publikationen

Species	Application	Title
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB	Cell Rep; Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.; Authors - Noah J Daniels ; KD Validated