CROP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CROP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14504-1-PBS

Synonyme

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

---

• 

  WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14504-1-AP (same clone as 14504-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

14504-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CROP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CROP fusion protein Ag5961
• Vollständiger Name: cisplatin resistance-associated overexpressed protein
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC056409
• Gene symbol: CROP
• Gene ID (NCBI): 51747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.