C-Reactive Protein/CRP Polyklonaler Antikörper

C-Reactive Protein/CRP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 24175-1-AP

Synonyme

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

---

• 

  WB analysis using 24175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24175-1-AP (C-Reactive Protein/CRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of serum from mouse injected with bacteria using 24175-1-AP

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 24175-1-AP (C-Reactive Protein/CRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 24175-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24175-1-AP (C-Reactive Protein/CRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 24175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24175-1-AP (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 24175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24175-1-AP (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: rat plasma, mit Bakterien injiziertes Serum aus der Maus
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

24175-1-AP bindet in WB, IHC, ELISA C-Reactive Protein/CRP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C-Reactive Protein/CRP fusion protein Ag21466
• Vollständiger Name: C-reactive protein, pentraxin-related
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC125135
• Gene symbol: CRP
• Gene ID (NCBI): 1401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Publikationen

Species	Application	Title
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
human	WB	J Proteome Res; Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.; Authors - Yong Zhou
human	WB	Biomed Mater; Reversing cytotoxicity of uric acid by supramolecular encapsulation with acyclic cucurbit[n]uril.; Authors - Lingguang Kong
mouse	IHC	Biochem Biophys Res Commun; In vitro and in vivo study of andrographolide nanoparticles for the treatment of Mycoplasma pneumoniae pneumonia; Authors - Liling Wang
		Exp Ther Med; TLR4 gene in the regulation of periodontitis and its molecular mechanism.; Authors - Weijuan Qi
rat	WB	ACS Omega; TMT Labeling Reveals the Effects of Exercises on the Proteomic Characteristics of the Subcutaneous Adipose Tissue of Growing High-Fat-Diet-Fed Rats; Authors - Ge Song