C-Reactive Protein/CRP Monoklonaler Antikörper

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C-Reactive Protein/CRP Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F10A11

Kat-Nr. : 66250-1-PBS

Synonyme

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

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  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

  Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66250-1-Ig (same clone as 66250-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 66250-1-Ig (same clone as 66250-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66250-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA C-Reactive Protein/CRP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C-Reactive Protein/CRP fusion protein Ag9883
• Vollständiger Name: C-reactive protein, pentraxin-related
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC020766
• Gene symbol: CRP
• Gene ID (NCBI): 1401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.