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CRTAC1 Polyklonaler Antikörper

CRTAC1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30251-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRTAC1 Polyclonal antibody (30251-1-AP)

    IHC staining of mouse brain using 30251-1-AP (same clone as 30251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CRTAC1 Polyclonal antibody (30251-1-AP)

    IHC staining of rat brain using 30251-1-AP (same clone as 30251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30251-1-PBS bindet in WB, IHC, Indirect ELISA CRTAC1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CRTAC1 fusion protein Ag32963
Vollständiger Name cartilage acidic protein 1
Berechnetes Molekulargewicht 661 aa, 71 kDa
Beobachtetes Molekulargewicht 71-100 kDa
GenBank-ZugangsnummerBC034245
Gene symbol CRTAC1
Gene ID (NCBI) 55118
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).