CRTAC1 Monoklonaler Antikörper

CRTAC1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A11E2

Kat-Nr. : 68276-1-PBS

Synonyme

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320

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  WB analysis using 68276-1-Ig (same clone as 68276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68276-1-Ig (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68276-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68276-1-Ig (same clone as 68276-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAC1 antibody (68276-1-Ig, Clone: 2A11E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68276-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68276-1-PBS bindet in WB, IF/ICC, Indirect ELISA CRTAC1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRTAC1 fusion protein Ag32813
• Vollständiger Name: cartilage acidic protein 1
• Berechnetes Molekulargewicht: 661 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC034245
• Gene symbol: CRTAC1
• Gene ID (NCBI): 55118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRTAC1 (cartilage acidic protein 1) is also named as ASPIC1 and CEP68. CRTAC1 is an extracellular matrix protein of human chondrogenic tissue. CRTAC1 promotes cell proliferation, migration and extracellular matrix regeneration and remodeling in primary human fibroblasts (PMID:32084494). CRTAC1 expression is decreased in tissue samples of bladder urothelial carcinoma (PMID:34818994). CRTAC1 overexpression promotes the pyroptosis of human lens epithelial cells by facilitating the reactive oxygen species (ROS) production in the formation of cataract (PMID:32838966). CRTAC1 was the most compelling and robust biomarker for osteoarthritis severity and progression (PMID:35924962).