CRTAM/CD355 Rekombinanter Antikörper

CRTAM/CD355 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250055A1

Kat-Nr. : 98420-3-RR

Synonyme

CD355, Class-I MHC-restricted T-cell-associated molecule, Crtam, Cytotoxic and regulatory T-cell molecule

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  FC experiment of mouse splenocytes using 98420-3-RR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CRTAM/CD355 Rabbit RecAb (98420-3-RR, Clone: 250055A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD8a (53-6.7) (APC-65069, Clone: 53-6.7). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98420-3-RR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CRTAM/CD355 Rabbit RecAb (98420-3-RR, Clone: 250055A1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse Crtam(CD355 ) Rabbit RecAb (98420-3-RR, Clone:250055A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98420-3-RR bindet in FC CRTAM/CD355 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CRTAM/CD355 fusion protein Eg2691
• Vollständiger Name: cytotoxic and regulatory T cell molecule
• Berechnetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: NM_001281954.1
• Gene symbol: Crtam
• Gene ID (NCBI): 54698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).