CRTAM/CD355 Rekombinanter Antikörper

CRTAM/CD355 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

241280B12

Kat-Nr. : FITC-98157

Synonyme

CRTAM, 241280B12, CD355, Class-I MHC-restricted T-cell-associated molecule, Cytotoxic and regulatory T-cell molecule

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  FC experiment of human PBMCs using FITC-98157

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD8 and 5 ul FITC Plus Anti-Human CRTAM/CD355 Rabbit RecAb (FITC-98157, Clone: 241280B12). Cells were not fixed.

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  FC experiment of human PBMCs using FITC-98157

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CRTAM/CD355 Rabbit RecAb (FITC-98157, Clone: 241280B12) (red), or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9)(blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CRTAM/CD355 Rabbit RecAb (FITC-98157, Clone: 241280B12) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98157 bindet in FC CRTAM/CD355 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CRTAM/CD355 fusion protein Eg1541
• Vollständiger Name: cytotoxic and regulatory T cell molecule
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_019604.4
• Gene symbol: CRTAM
• Gene ID (NCBI): 56253
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).