CRTAP Polyklonaler Antikörper

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• KD/KO Validated

CRTAP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15724-1-AP

Synonyme

Cartilage-associated protein, CASP, LEPREL3

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  WB analysis using 15724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15724-1-AP

  WB result of CRTAP antibody (15724-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRTAP transfected HeLa cells.

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  WB analysis of HeLa using 15724-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15724-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 15724-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 15724-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 15724-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 15724-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15724-1-AP

  IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.

• 

  IHC staining of human kidney using 15724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Kolongewebe, humanes Magengewebe, NIH/3T3-Zellen, ROS1728-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

15724-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CRTAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRTAP fusion protein Ag8459
• Vollständiger Name: cartilage associated protein
• Berechnetes Molekulargewicht: 46.5 kDa
• Beobachtetes Molekulargewicht: 43-47 kDa
• GenBank-Zugangsnummer: BC008745
• Gene symbol: CRTAP
• Gene ID (NCBI): 10491
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Med Oncol; Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer; Authors - Liangshun Zhang
human	WB	Nat Commun; The structural basis for the collagen processing by human P3H1/CRTAP/PPIB ternary complex; Authors - Wenguo Li