CRX Polyklonaler Antikörper

CRX Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12047-1-AP

Synonyme

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, diencephalon/mesencephalon homeobox 1, DMBX1, LCA7, OTX3

---

Galerie der Validierungsdaten

• 

  WB analysis of Y79 using 12047-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12047-1-AP (CRX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12047-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12047-1-AP (CRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 12047-1-AP

  IP Result of anti-CRX (IP:12047-1-AP, 4ug; Detection:12047-1-AP 1:500) with Y79 cells lysate 1920ug.

• 

  IHC staining of mouse eye using 12047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 12047-1-AP (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12047-1-AP (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12047-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CRX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRX fusion protein Ag2677
• Vollständiger Name: cone-rod homeobox
• Berechnetes Molekulargewicht: 299 aa, 32 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC016664
• Gene symbol: CRX
• Gene ID (NCBI): 1406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

Publikationen

Species	Application	Title
human	WB, CoIP	J Neurosci; The transcription factor GTF2IRD1 regulates the topology and function of photoreceptors by modulating photoreceptor gene expression across the retina.; Authors - Tomohiro Masuda
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian