Cryptochrome 1 Rekombinanter Antikörper

Cryptochrome 1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240520B12

Kat-Nr. : 83602-5-RR

Synonyme

CRY1, Cryptochrome-1, CRY 1, 240520B12

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  WB analysis using 83602-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83602-5-RR (CRY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83602-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83602-5-RR against Human Cryptochrome 1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83602-5-RR bindet in WB, ELISA Cryptochrome 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cryptochrome 1 fusion protein Ag4270
• Vollständiger Name: cryptochrome 1 (photolyase-like)
• Berechnetes Molekulargewicht: 586 aa, 66 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC030519
• Gene symbol: CRY1
• Gene ID (NCBI): 1407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481).