Alpha B Crystallin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Alpha B Crystallin Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15808-1-AP

Synonyme

Alpha B Crystallin, Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component

---

Galerie der Validierungsdaten

• 

  WB analysis using 15808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of U2OS using 15808-1-AP

  IP result of anti-Alpha B Crystallin(IP:15808-1-AP, 4ug; Detection:15808-1-AP 1:500) with U2OS cells lysate 1800 ug.

• 

  IHC staining of mouse eye using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 15808-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe, U2OS-Zellen
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

15808-1-AP bindet in WB, IP, IHC, IF, ELISA Alpha B Crystallin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha B Crystallin fusion protein Ag8543
• Vollständiger Name: crystallin, alpha B
• Berechnetes Molekulargewicht: 175 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC007008
• Gene symbol: CRYAB
• Gene ID (NCBI): 1410
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.

Publikationen

Species	Application	Title
mouse	IF	Brief Bioinform; iSMNN: batch effect correction for single-cell RNA-seq data via iterative supervised mutual nearest neighbor refinement.; Authors - Yuchen Yang
human	WB	Oxid Med Cell Longev; Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach.; Authors - Daniele Vergara
human,mouse	WB	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
human	WB	Front Cell Dev Biol; A Novel Ferroptosis-Related Prognostic Signature Reveals Macrophage Infiltration and EMT Status in Bladder Cancer.; Authors - Yilin Yan ; KD Validated
fish	ELISA	Food Res Int; Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).; Authors - Lihong Ge
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Deficiency of heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21cip1 expression.; Authors - Xiukun Cui