Citrate synthase Polyklonaler Antikörper

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• KD/KO Validated

Citrate synthase Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

74

Rezensionen (1)

Kat-Nr. : 16131-1-AP

Synonyme

Citrate synthase, CS

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 16131-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16131-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16131-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16131-1-AP

  IP Result of anti-Citrate synthase (IP:16131-1-AP, 4ug; Detection:16131-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of human liver cancer using 16131-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16131-1-AP (Citrate synthase antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16131-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16131-1-AP (Citrate synthase antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16131-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16131-1-AP (Citrate synthase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16131-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16131-1-AP (Citrate synthase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 63 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16131-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA Citrate synthase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Citrate synthase fusion protein Ag9117
• Vollständiger Name: citrate synthase
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC010106
• Gene symbol: CS
• Gene ID (NCBI): 1431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.; Authors - Miranda Y Fong
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	IF	ACS Cent Sci; A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species; Authors - Cong Tang
human	IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
mouse	WB,IF	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RAHUL (Verified Customer) (07-03-2019)

Works very good with human cell lysates. 1:1000 dilution in 5% milk; 1 hr incubation at RT.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: DU145 prostate cancer cells