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Citrate synthase Polyklonaler Antikörper
Citrate synthase Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16131-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, HepG2-Zellen, MDA-MB-231-Zellen, Mausherzgewebe |
Erfolgreiche IP | Mausherzgewebe |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:100-1:400 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 73 publications below |
IHC | See 4 publications below |
IF | See 10 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Produktinformation
16131-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Citrate synthase und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, hamster, Huhn, Kaninchen, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Citrate synthase fusion protein Ag9117 |
Vollständiger Name | citrate synthase |
Berechnetes Molekulargewicht | 466 aa, 52 kDa |
Beobachtetes Molekulargewicht | 45-50 kDa |
GenBank-Zugangsnummer | BC010106 |
Gene symbol | Citrate Synthase |
Gene ID (NCBI) | 1431 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Citrate synthase antibody 16131-1-AP | Protokoll herunterladen |
IHC protocol for Citrate synthase antibody 16131-1-AP | Protokoll herunterladenl |
IF protocol for Citrate synthase antibody 16131-1-AP | Protokoll herunterladen |
IP protocol for Citrate synthase antibody 16131-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration | ||
Nat Cell Biol Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis. | ||
ACS Cent Sci A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sami Elamin Mustafa (Verified Customer) (01-31-2025) | The antibody works nicely giving clear sharp bands as expected.
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FH RAHUL (Verified Customer) (07-03-2019) | Works very good with human cell lysates. 1:1000 dilution in 5% milk; 1 hr incubation at RT.
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