Citrate synthase Rekombinanter Antikörper

Citrate synthase Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251433D2

Kat-Nr. : 86549-1-PBS

Synonyme

CS, CISY, Citrate, Citrate synthase, mitochondrial, EC:2.3.3.1

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  WB analysis using 86549-1-RR (same clone as 86549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 86549-1-RR (same clone as 86549-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86549-1-RR (same clone as 86549-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 86549-1-RR (same clone as 86549-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02513-1

  The mean Citrate synthase concentration was determined to be 135.1 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02513-1

  Cytometric bead array standard curve of MP02513-1, Citrate synthase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86549-2-PBS. Detection antibody: 86549-1-PBS. Standard: Ag9117. Range: 6.25-200 ng/mL.

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  Affinity and Kinetic Characterization of 86549-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86549-1-RR against Human Citrate synthase were performed. The affinity constant is 0.995 nM.

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Geprüfte Anwendungen

Produktinformation

86549-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA Citrate synthase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Citrate synthase fusion protein Ag9117
• Vollständiger Name: citrate synthase
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC010106
• Gene symbol: Citrate Synthase
• Gene ID (NCBI): 1431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038).