CSDA Polyklonaler Antikörper

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• KD/KO Validated

CSDA Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27785-1-PBS

Synonyme

cold shock domain protein A, Cold shock domain-containing protein A, DBPA, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB

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  WB analysis of KYSE-30 using 27785-1-AP (same clone as 27785-1-PBS)

  WB result of CSDA antibody (27785-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells. This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  WB analysis using 27785-1-AP (same clone as 27785-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27785-1-AP (CSDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27785-1-AP (same clone as 27785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 27785-1-AP (same clone as 27785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 27785-1-AP (same clone as 27785-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSDA antibody (27785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27785-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CSDA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSDA fusion protein Ag26839
• Vollständiger Name: cold shock domain protein A
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC008801
• Gene symbol: CSDA
• Gene ID (NCBI): 8531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).