CSDC2 Rekombinanter Antikörper

CSDC2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241031A11

Kat-Nr. : 83886-6-RR

Synonyme

RNA-binding protein PIPPin, RNA binding protein PIPPin, PIPPIN, Cold shock domain-containing protein C2, 241031A11

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  IF Staining of HeLa using 83886-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSDC2 antibody (83886-6-RR, Clone: 241031A11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-2 OS using 83886-6-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug CSDC2 Recombinant antibody (83886-6-RR, Clone:241031A11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83886-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83886-6-RR against Human CSDC2 were performed. The affinity constant is 0.256 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): U-2 OS

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83886-6-RR bindet in IF/ICC, FC (Intra), ELISA CSDC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CSDC2 fusion protein Ag16216
• Vollständiger Name: cold shock domain containing C2, RNA binding
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• GenBank-Zugangsnummer: BC067113
• Gene symbol: CSDC2
• Gene ID (NCBI): 27254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).