CSDE1 Polyklonaler Antikörper

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• KD/KO Validated

CSDE1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13319-1-AP

Synonyme

CSDE1, D1S155E, KIAA0885, N ras upstream gene protein, NRU, Protein UNR, RP5 1000E10.3, UNR

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Galerie der Validierungsdaten

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  WB analysis using 13319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13319-1-AP (CSDE1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 13319-1-AP

  WB result of CSDE1 antibody (13319-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSDE1 transfected A431 cells.

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  IHC staining of human liver cancer using 13319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13319-1-AP (CSDE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13319-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSDE1 antibody (13319-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

13319-1-AP bindet in WB, IHC, IF, ELISA CSDE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSDE1 fusion protein Ag4173
• Vollständiger Name: cold shock domain containing E1, RNA-binding
• Berechnetes Molekulargewicht: 798 aa, 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC032446
• Gene symbol: CSDE1
• Gene ID (NCBI): 7812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.

Publikationen

Species	Application	Title
human	WB	Nat Protoc; Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.; Authors - Joel I Perez-Perri
human	WB	Nat Commun; Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture.; Authors - Joel I Perez-Perri
human,mouse	WB	Blood; Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts.; Authors - Horos Rastislav R ; KD Validated
human	WB	Viruses; Upstream of N-Ras (Unr/CSDE1) Interacts with NCp7 and Gag, Modulating HIV-1 IRES-Mediated Translation Initiation; Authors - Nedal Taha ; KD Validated