CSDE1 Polyklonaler Antikörper

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• KD/KO Validated

CSDE1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13319-1-PBS

Synonyme

UNR, NRU, N-ras upstream gene protein, KIAA0885, Cold shock domain-containing protein E1

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  WB analysis using 13319-1-AP (same clone as 13319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13319-1-AP (CSDE1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 13319-1-AP (same clone as 13319-1-PBS)

  WB result of CSDE1 antibody (13319-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSDE1 transfected A431 cells. This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13319-1-AP (same clone as 13319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13319-1-AP (CSDE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13319-1-AP (same clone as 13319-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSDE1 antibody (13319-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13319-1-PBS bindet in WB, IHC, IF/ICC, ELISA CSDE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSDE1 fusion protein Ag4173
• Vollständiger Name: cold shock domain containing E1, RNA-binding
• Berechnetes Molekulargewicht: 798 aa, 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC032446
• Gene symbol: CSDE1
• Gene ID (NCBI): 7812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.