CSE1L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CSE1L Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 22219-1-AP

Synonyme

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2

---

Galerie der Validierungsdaten

• 

  WB analysis using 22219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22219-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22219-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22219-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22219-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22219-1-AP

  IP result of anti-CSE1L(IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human placenta using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe, MCF-7-Zellen, NIH/3T3-Zellen, Ramos-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

22219-1-AP bindet in WB, IP, IHC, IF, ELISA CSE1L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSE1L fusion protein Ag17556
• Vollständiger Name: CSE1 chromosome segregation 1-like (yeast)
• Berechnetes Molekulargewicht: 971 aa, 110 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC109313
• Gene symbol: CSE1L
• Gene ID (NCBI): 1434
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio
human	WB,IHC	Sci Rep; CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.; Authors - Dong-Dong Cheng ; KD Validated
human	WB	J Biol Chem; RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.; Authors - Jackson B Trotman
human,mouse	WB,IHC,IF	Int J Oncol; Butyrate supplementation regulates expression of chromosome segregation 1‑like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer.; Authors - Chun-Chao Chang ; KD Validated
human	WB	J Cancer; CSE1L, as a novel prognostic marker, promotes pancreatic cancer proliferation by regulating the AKT/mTOR signaling pathway.; Authors - Xiao Zhang ; KD Validated