CSE1L Polyklonaler Antikörper

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CSE1L Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22219-1-PBS

Synonyme

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• 

  WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 22219-1-AP (same clone as 22219-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 22219-1-AP (same clone as 22219-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW264.7 using 22219-1-AP (same clone as 22219-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)

  IP result of anti-CSE1L (IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22219-1-PBS bindet in WB, IHC, IP, Indirect ELISA CSE1L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSE1L fusion protein Ag17556
• Vollständiger Name: CSE1 chromosome segregation 1-like (yeast)
• Berechnetes Molekulargewicht: 971 aa, 110 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC109313
• Gene symbol: CSE1L
• Gene ID (NCBI): 1434
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.