CSF1R/CD115 Polyklonaler Antikörper

CSF1R/CD115 Polyklonal Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 25949-1-AP

Synonyme

CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor

---

• 

  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of THP-1 using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF1R/CD115 antibody (25949-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Produktinformation

25949-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA CSF1R/CD115 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSF1R/CD115 fusion protein Ag22845
• Vollständiger Name: colony stimulating factor 1 receptor
• Berechnetes Molekulargewicht: 972 aa, 108 kDa
• GenBank-Zugangsnummer: BC047521
• Gene symbol: CSF1R/CD115
• Gene ID (NCBI): 1436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Publikationen

Species	Application	Title
human	IHC	Cancers (Basel); Targeting CSF1R Alone or in Combination with PD1 in Experimental Glioma.; Authors - Justyna M Przystal
human	IHC	Arthritis Res Ther; Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.; Authors - Ping Li
human	IHC	CNS Neurosci Ther; A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma; Authors - Wenhua Fan
rat	WB	J Nat Med; Insight of action mechanism of Astragaloside IV for relieving of cerebral ischemic injury in a rat model of middle cerebral artery occlusion reperfusion via proteomics and network pharmacology; Authors - Xin-Hua Zhu
mouse	WB	J Transl Med; ITIH5-mediated fibroblast/macrophage crosstalk exacerbates cardiac remodelling after myocardial infarction; Authors - Yirong Wu
mouse	WB	Toxics; CuO-NPs Induce Apoptosis and Functional Impairment in BV2 Cells Through the CSF-1R/PLCγ2/ERK/Nrf2 Pathway; Authors - Linhui Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Jaouhara (Verified Customer) (12-05-2023)

Used in synovium PFA-fixed slides to detect macrophages. Works well.

• Applications: Immunohistochemistry