CSF1R Polyklonaler Antikörper

CSF1R Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 25949-1-AP

Synonyme

C FMS, CD115, CSF 1 R, CSF1R, CSFR, FIM2, FMS, Proto oncogene c Fms

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Galerie der Validierungsdaten

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  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Cerebellum-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

25949-1-AP bindet in WB, IHC, IF, ELISA CSF1R und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSF1R fusion protein Ag22845
• Vollständiger Name: colony stimulating factor 1 receptor
• Berechnetes Molekulargewicht: 972 aa, 108 kDa
• GenBank-Zugangsnummer: BC047521
• Gene symbol: CSF1R
• Gene ID (NCBI): 1436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Cancers (Basel); Targeting CSF1R Alone or in Combination with PD1 in Experimental Glioma.; Authors - Justyna M Przystal
human	IHC	Arthritis Res Ther; Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.; Authors - Ping Li
human	IHC	CNS Neurosci Ther; A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma; Authors - Wenhua Fan
mouse	WB	Exploration (Beijing); Microglial targeted therapy relieves cognitive impairment caused by Cntnap4 deficiency; Authors - Wenlong Zhang
human	IHC	Front Genet; Human placenta-based genome-wide mRNA sequencing to identify TEK/IGF1/CSF1/ANGPT2 as crucial segments in the pathogenesis of pre-eclampsia; Authors - Lifeng Wang
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Used in synovium PFA-fixed slides to detect macrophages. Works well.

• Applications: Immunohistochemistry