CSF1R/CD115 Rekombinanter Antikörper

CSF1R/CD115 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242213B1

Kat-Nr. : 84919-1-RR

Synonyme

Csf1r, Csfmr, CSF-1 receptor, CSF 1R, CD115

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  WB analysis using 84919-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84919-1-RR

  J774A.1 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 84919-1-RR

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84919-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CSF1R/CD115 antibody (84919-1-RR, Clone: 242213B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84919-1-RR bindet in WB, IF/ICC, ELISA CSF1R/CD115 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: colony stimulating factor 1 receptor
• Berechnetes Molekulargewicht: 109 kDa
• Beobachtetes Molekulargewicht: 110-170 kDa
• GenBank-Zugangsnummer: NM_001037859.2
• Gene symbol: CSF1R/CD115
• Gene ID (NCBI): 12978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).