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CSF1R/CD115 Rekombinanter Antikörper

CSF1R/CD115 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242213E1

Kat-Nr. : 84919-4-RR

Synonyme

Csf1r, 242213E1, CD115, CSF 1R, CSF-1 receptor

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using CSF1R/CD115 Recombinant antibody (84919-4-RR)

    WB analysis of RAW 264.7 using 84919-4-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84919-4-RR (CSF1R/CD115 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CSF1R/CD115 Recombinant monoclonal antibody (84919-4-RR)

    IF Staining of RAW 264.7 using 84919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CSF1R/CD115 antibody (84919-4-RR, Clone: 242213E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRAW 264.7-Zellen
Erfolgreiche Detektion in IF/ICCRAW 264.7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

84919-4-RR bindet in WB, IF/ICC, ELISA CSF1R/CD115 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CSF1R/CD115 fusion protein Eg1816
Vollständiger Name colony stimulating factor 1 receptor
Berechnetes Molekulargewicht109 kDa
Beobachtetes Molekulargewicht165 kDa
GenBank-ZugangsnummerNM_001037859.2
Gene symbol CSF1R/CD115
Gene ID (NCBI) 12978
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CSF1R/CD115 antibody 84919-4-RRProtokoll herunterladen
IF protocol for CSF1R/CD115 antibody 84919-4-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen