IL-3RB/CD131 Rekombinanter Antikörper

IL-3RB/CD131 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241954C1

Kat-Nr. : 98232-1-RR

Synonyme

241954C1, Aic2b, CD131, Csf2rb, Csf2rb1

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  FC experiment of mouse bone marrow cells using 98232-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IL-3RB/CD131 Rabbit RecAb (98232-1-RR, Clone: 241954C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98232-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IL-3RB/CD131 Rabbit RecAb (98232-1-RR, Clone: 241954C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98232-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98232-1-RR against Mouse IL-3RB/CD131 were performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 98232-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98232-1-RR against Mouse IL-3RB/CD131 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98232-1-RR bindet in FC IL-3RB/CD131 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-3RB/CD131 fusion protein Eg2173
• Vollständiger Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• Berechnetes Molekulargewicht: 99kDa
• GenBank-Zugangsnummer: NM_007780.4
• Gene symbol: IL-3RB/CD131
• Gene ID (NCBI): 12983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD131, also known as IL-3RB, IL-5RB, colony stimulating factor 2 receptor subunit beta (CSF2RB), or cytokine receptor common subunit beta, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 and IL-5 (PMID: 36532080). It is expressed predominantly by hematopoietic cells but also by non-hematopoietic cells (PMID: 10582335). CD131 mediates intracellular signaling by JAK2, leading to phosphorylation of CD131 and triggering signaling pathways such as STAT5, MAPK and PI3-Kinase/AKT which regulate cell survival, proliferation and function (PMID: 23535386).