G-CSFR Rekombinanter Antikörper

G-CSFR Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242246A5

Kat-Nr. : 84827-2-PBS

Synonyme

242246A5, CD114, CSF3R, G CSF R, G CSF receptor

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  WB analysis of THP-1 using 84827-2-RR (same clone as 84827-2-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

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  WB analysis using 84827-2-RR (same clone as 84827-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01609-3

  Sandwich ELISA standard curve of MP01609-3, Human G-CSFR Recombinant Matched Antibody Pair - PBS only. 84827-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2821. 84827-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP01609-3

  The mean G-CSFR concentration was determined to be 7.76 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load.

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  IHC staining of human placenta using 84827-2-RR (same clone as 84827-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84827-2-RR (G-CSFR antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84827-2-RR (same clone as 84827-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84827-2-RR (G-CSFR antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84827-2-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test G-CSFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: G-CSFR fusion protein Eg2821
• Vollständiger Name: colony stimulating factor 3 receptor (granulocyte)
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: NM_000760.3
• Gene symbol: G-CSFR
• Gene ID (NCBI): 1441
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.