Placental lactogen Rekombinanter Antikörper

Placental lactogen Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241625E1

Kat-Nr. : 84361-2-RR

Synonyme

CSH1, PL, CSH2, Chorionic somatomammotropin hormone 2, Choriomammotropin Alternative names Lactogen

---

• 

  WB analysis of human placenta using 84361-2-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84361-2-RR (CSH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 84361-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84361-2-RR (Placental lactogen antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84361-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84361-2-RR (Placental lactogen antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84361-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84361-2-RR against Human Placental lactogen were performed. The affinity constant is 2.58 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84361-2-RR bindet in WB, IHC, ELISA Placental lactogen und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Placental lactogen fusion protein Ag0201
• Vollständiger Name: chorionic somatomammotropin hormone 1 (placental lactogen)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC002717
• Gene symbol: CSH1
• Gene ID (NCBI): 1442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chorionic somatomammotropin hormone 1 (placental lactogen, CSH1), is a hormone with the molecular weight of 25 kDa. The data suggest that this protein may link with the Silver-Russell syndrome (SRS), a disease of pre- and postnatal growth restriction and a characteristic small, triangular face. SRS is also accompanied by other dysmorphic features including fifth finger clinodactyly and skeletal asymmetry.