Placental lactogen Polyklonaler Antikörper

Placental lactogen Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16326-1-AP

Synonyme

CSH2, Choriomammotropin, CS 2, CSB, CSH1

---

• 

  WB analysis using 16326-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16326-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 16326-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human placenta using 16326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Placental lactogen antibody (16326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 16326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Placental lactogen antibody (16326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

16326-1-AP bindet in WB, IHC, IF-P, ELISA Placental lactogen und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Placental lactogen fusion protein Ag9412
• Vollständiger Name: chorionic somatomammotropin hormone 2
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC022044
• Gene symbol: CSH2
• Gene ID (NCBI): 1443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus.

Publikationen

Species	Application	Title
	IHC	J Vet Med Sci; Localization of peptide hormones in the placentas of Bryde's (Balaenoptera brydei), sei (B. borealis), and common minke (B. acutorostrata) whales; Authors - Risa Aoki