CSNK1G1 Polyklonaler Antikörper

CSNK1G1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16384-1-PBS

Synonyme

---

• 

  WB analysis using 16384-1-AP (same clone as 16384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COS-7 using 16384-1-AP (same clone as 16384-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

  IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

16384-1-PBS bindet in WB, IHC, IP, Indirect ELISA CSNK1G1 und zeigt Reaktivität mit Affe, human

• Getestete Reaktivität: Affe, human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSNK1G1 fusion protein Ag9395
• Vollständiger Name: casein kinase 1, gamma 1
• Berechnetes Molekulargewicht: 422 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC017236
• Gene symbol: CSNK1G1
• Gene ID (NCBI): 53944
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.