CSNK2A1 Monoklonaler Antikörper

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• KD/KO Validated

CSNK2A1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1D5E8

Kat-Nr. : 68200-1-Ig

Synonyme

1D5E8, CK II alpha, CK2a, CK2A1, CK2α

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  WB analysis using 68200-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 68200-1-Ig

  WB result of CSNK2A1 antibody (68200-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells.

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  WB analysis of rabbit heart using 68200-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 68200-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 68200-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen, Kaninchenherzgewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

68200-1-Ig bindet in WB, IF/ICC, IP, ELISA CSNK2A1 und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CSNK2A1 fusion protein Ag29062
• Vollständiger Name: casein kinase 2, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC011668
• Gene symbol: CSNK2A1
• Gene ID (NCBI): 1457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Publikationen

Species	Application	Title
human	WB,IP	F1000Res; A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence; Authors - Riham Ayoubi ; KO Validated