CSNK2A1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CSNK2A1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5E8

Kat-Nr. : 68200-1-PBS

Synonyme

1D5E8, CK II alpha, CK2a, CK2A1, CK2α

---

• 

  WB analysis using 68200-1-Ig (same clone as 68200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68200-1-Ig (same clone as 68200-1-PBS)

  WB result of CSNK2A1 antibody (68200-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 68200-1-Ig (same clone as 68200-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 68200-1-Ig (same clone as 68200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68200-1-Ig (same clone as 68200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68200-1-PBS bindet in WB, IF/ICC, Indirect ELISA CSNK2A1 und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CSNK2A1 fusion protein Ag29062
• Vollständiger Name: casein kinase 2, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC011668
• Gene symbol: CSNK2A1
• Gene ID (NCBI): 1457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.