CSNK2A1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CSNK2A1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240862A7

Kat-Nr. : 83805-4-RR

Synonyme

240862A7, CK II alpha, CK2a, CK2A1, CK2α

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  WB analysis using 83805-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83805-4-RR (CSNK2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 83805-4-RR

  WB result of CSNK2A1 antibody (83805-4-RR; 1:200000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells.

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  IF Staining of HeLa using 83805-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 83805-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 83805-4-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CSNK2A1 Recombinant antibody (83805-4-RR, Clone:240862A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83805-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83805-4-RR against Human CSNK2A1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, A431-Zellen, A549-Zellen, C6-Zellen, NIH/3T3-Zellen, Raji-Zellen, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83805-4-RR bindet in WB, IF/ICC, FC (Intra), ELISA CSNK2A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CSNK2A1 fusion protein Ag1452
• Vollständiger Name: casein kinase 2, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC011668
• Gene symbol: CSNK2A1
• Gene ID (NCBI): 1457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.