CSNK2A2 Polyklonaler Antikörper

CSNK2A2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10606-1-AP

Synonyme

Casein kinase II subunit alpha', CK II alpha', CK2A2, CSNK2A1, CSNK2A2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10606-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10606-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10606-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10606-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10606-1-AP

  IP result of anti-CSNK2A2 (IP:10606-1-AP, 4ug; Detection:10606-1-AP 1:1000) with Jurkat cells lysate 2800 ug.

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  IF Staining of HepG2 using 10606-1-AP

  Immunofluorescent analysis of HepG2 cells using 10606-1-AP (CSNK2A2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HT-1080.Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

10606-1-AP bindet in WB, IP, IHC, IF, ELISA CSNK2A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSNK2A2 fusion protein Ag0877
• Vollständiger Name: casein kinase 2, alpha prime polypeptide
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC008812
• Gene symbol: CSNK2A2
• Gene ID (NCBI): 1459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2A2(Casein kinase II subunit alpha') is also named as CK2A3, CK2A2, CSNK2A1, CK2 and belongs to the protein kinase superfamily. It can function as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage during mitosis. It also can protect against ionizing radiation-induced apoptosis partly by phosphorylating and activating SIRT1. This protein has molecular weights between 35 kDa and 44 kDa(PMID:8622692).

Publikationen

Species	Application	Title
mouse	WB,IHC	Acta Neuropathol Commun; CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease.; Authors - Dahyun Yu
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
mouse	WB	Cell Rep; Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease; Authors - Rachel H Mansky ; KD Validated