CSNK2B Monoklonaler Antikörper

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• KD/KO Validated

CSNK2B Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5A6

Kat-Nr. : 67866-1-PBS

Synonyme

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

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  WB analysis using 67866-1-Ig (same clone as 67866-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67866-1-Ig (same clone as 67866-1-PBS)

  WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67866-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CSNK2B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CSNK2B fusion protein Ag19180
• Vollständiger Name: casein kinase 2, beta polypeptide
• Berechnetes Molekulargewicht: 215 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC112017
• Gene symbol: CSNK2B
• Gene ID (NCBI): 1460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.