CSNK2B Polyklonaler Antikörper

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• KD/KO Validated

CSNK2B Polyklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20234-1-PBS

Synonyme

CSNK2B-specific, Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B

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  WB analysis using 20234-1-AP (same clone as 20234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20234-1-AP (same clone as 20234-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 20234-1-AP (same clone as 20234-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 20234-1-AP (same clone as 20234-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20234-1-AP (same clone as 20234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20234-1-AP (same clone as 20234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 20234-1-AP (same clone as 20234-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CSNK2B-specific (20234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20234-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA CSNK2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: casein kinase 2, beta polypeptide
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_001320
• Gene symbol: CSNK2B
• Gene ID (NCBI): 1460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.