CSPG4 Rekombinanter Antikörper

CSPG4 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240733C9

Kat-Nr. : 83716-1-RR

Synonyme

NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP

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  WB analysis of A375 using 83716-1-RR

  A375 cells were subjected to SDS PAGE followed by western blot with 83716-1-RR (CSPG4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

83716-1-RR bindet in WB, IHC, ELISA CSPG4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: chondroitin sulfate proteoglycan 4
• Berechnetes Molekulargewicht: 251kd
• Beobachtetes Molekulargewicht: 250-310 kDa
• GenBank-Zugangsnummer: NM_001897.5
• Gene symbol: CSPG4
• Gene ID (NCBI): 1464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 may regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. It modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. CSPG4 functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. It promotes retraction fiber formation and cell polarization through Rho GTPase activation and stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. CSPG4 activates FAK and ERK1/ERK2 signaling cascades. The antibody is specific to CSPG4.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Biol Reprod; CSPG4 involvement in endometrial decidualization contributes to the pathogenesis of preeclampsia; Authors - Tianying Zhang