CSRP2 Polyklonaler Antikörper

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• KD/KO Validated

CSRP2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10892-2-PBS

Synonyme

CRP2, Cysteine and glycine-rich protein 2, Cysteine rich protein 2, Cysteine-rich protein 2, LMO5

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  WB analysis using 10892-2-AP (same clone as 10892-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10892-2-AP (same clone as 10892-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 10892-2-AP (same clone as 10892-2-PBS)

  IP result of anti-CSRP2 (IP:10892-2-AP, 4ug; Detection:10892-2-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10892-2-AP (same clone as 10892-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10892-2-AP (CSRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10892-2-PBS bindet in WB, IHC, IP, Indirect ELISA CSRP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSRP2 fusion protein Ag1338
• Vollständiger Name: cysteine and glycine-rich protein 2
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000992
• Gene symbol: CSRP2
• Gene ID (NCBI): 1466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSRP2 (Cysteine and glycine-rich protein 2) is a member of the LIM-only family of cysteine-rich proteins and contains two LIM domains, which has been recently implicated in the progression and metastasis of a variety of cancers. CSRP2 is functionally linked to the activity of TGF-b1 in hepatic stellate cells (HSC), derivatives thereof, and in differentiated VSMC.CSRP2 knockdown signifcantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells (PMID: 33042270). CSRP2 is a short (21 kDa) two LIM domain-containing protein, which is upregulated in invasive breast cancer cells, and localizes along the protrusive actin core of invadopodium1 (PMID: 29976963, 16735029).