Cystatin SN/CST1 Rekombinanter Antikörper

Cystatin SN/CST1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242888B3

Kat-Nr. : 85403-1-PBS

Synonyme

CST1, Cystain SA I, Cystain-SA-I, Cystatin 1, cystatin SN

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  WB analysis using 85403-1-RR (same clone as 85403-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 85403-1-RR (CST1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85403-1-PBS in a different storage buffer formulation.

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  WB analysis of human saliva using 85403-1-RR (same clone as 85403-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 85403-1-RR (Cystatin SN/CST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85403-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01926-3

  Sandwich ELISA standard curve of MP01926-3, Human Cystatin SN/CST1 Recombinant Matched Antibody Pair - PBS only. 85403-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8862. 85403-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-10 ng/mL

• 

  Sample test of MP01926-3

  Urine of seven individual healthy human donors was measured. The human Cystatin SN/CST1 concentration of detected samples was determined to be 0.70 ng/mL with a range of 0.16 - 2.31 ng/mL.

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  Sample test of MP01926-3

  Serum of eight individual healthy human donors was measured. The human Cystatin SN/CST1 concentration of detected samples was determined to be 5.27 ng/mL with a range of 2.95 - 7.40 ng/mL.

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  Affinity and Kinetic Characterization of 85403-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85403-1-RR against Human Cystatin SN/CST1 were performed. The affinity constant is 1.09 nM.

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Geprüfte Anwendungen

Produktinformation

85403-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test Cystatin SN/CST1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cystatin SN/CST1 fusion protein Ag8862
• Vollständiger Name: cystatin SN
• Berechnetes Molekulargewicht: 141 aa, 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa
• GenBank-Zugangsnummer: BC021225
• Gene symbol: CST1
• Gene ID (NCBI): 1469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.