Cystatin A Polyklonaler Antikörper

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• KD/KO Validated

Cystatin A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 15962-1-AP

Synonyme

CSTA, Stefin-A, Cystatin-A, N-terminally processed, Cystatin-A, Cystatin AS

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  WB analysis of human saliva using 15962-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15962-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15962-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15962-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15962-1-AP (Cystatin A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 15962-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15962-1-AP (Cystatin A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15962-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cystatin A antibody (15962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Speichelgewebe, A549-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15962-1-AP bindet in WB, IHC, IF/ICC, ELISA Cystatin A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cystatin A fusion protein Ag8733
• Vollständiger Name: cystatin A (stefin A)
• Berechnetes Molekulargewicht: 98 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC010379
• Gene symbol: Cystatin A
• Gene ID (NCBI): 1475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatin A (CSTA) is a member of the cystatin superfamily, which are thiol proteinase inhibitors (TPI) that play crucial roles in regulating proteolytic activity. It is a small protein composed of 98 amino acids with a molecular weight of approximately 11 kDa. CSTA is primarily expressed in epithelial tissues, such as the skin and esophagus, where it serves as a precursor protein for the cornified cell envelope in keratinocytes.

Publikationen

Species	Application	Title
human	IHC	Cancer Res; Lung cancer diagnosis from proteomic analysis of preinvasive lesions.; Authors - Rahman S M Jamshedur SM
mouse	WB,IF	Biochem Biophys Res Commun; CSTA plays a role in osteoclast formation and bone resorption by mediating the DAP12/TREM2 pathway; Authors - Rui Wei ; KD Validated
human,mouse	WB	Cell Rep; TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation; Authors - Jihuan Hou