Cystatin A Rekombinanter Antikörper

Cystatin A Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242558F10

Kat-Nr. : 84953-4-RR

Synonyme

CSTA, 242558F10, Stefin-A, Cystatin-A, N-terminally processed, Cystatin-A

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  WB analysis of U2OS using 84953-4-RR

  U2OS cells were subjected to SDS PAGE followed by western blot with 84953-4-RR (CSTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84953-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84953-4-RR against Human Cystatin A were performed. The affinity constant is 0.286 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84953-4-RR bindet in WB, ELISA Cystatin A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cystatin A fusion protein Ag8733
• Vollständiger Name: cystatin A (stefin A)
• Berechnetes Molekulargewicht: 98 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC010379
• Gene symbol: Cystatin A
• Gene ID (NCBI): 1475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatin A (CSTA) is a member of the cystatin superfamily, which are thiol proteinase inhibitors (TPI) that play crucial roles in regulating proteolytic activity. It is a small protein composed of 98 amino acids with a molecular weight of approximately 11 kDa. CSTA is primarily expressed in epithelial tissues, such as the skin and esophagus, where it serves as a precursor protein for the cornified cell envelope in keratinocytes.