CSTF3 Polyklonaler Antikörper

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• KD/KO Validated

CSTF3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24290-1-AP

Synonyme

CF-1 77 kDa subunit, CSTF 77, CstF-77

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  WB analysis using 24290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24290-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 24290-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 24290-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 24290-1-AP

  IP result of anti-CSTF3 (IP:24290-1-AP, 4ug; Detection:24290-1-AP 1:500) with A431 cells lysate 1200ug.

• 

  IHC staining of human stomach cancer using 24290-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24290-1-AP (CSTF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

24290-1-AP bindet in WB, IHC, IF, IP, ELISA CSTF3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSTF3 fusion protein Ag17930
• Vollständiger Name: cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
• Berechnetes Molekulargewicht: 717 aa, 83 kDa
• Beobachtetes Molekulargewicht: 77-83 kDa
• GenBank-Zugangsnummer: BC059948
• Gene symbol: CSTF3
• Gene ID (NCBI): 1479
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IF,IP	iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min ; KD Validated
human	WB	Comput Struct Biotechnol J; Alternative polyadenylation regulates the translation of metabolic and inflammation-related proteins in adipose tissue of gestational diabetes mellitus; Authors - Bingnan Chen
human	IF	Cell Death Dis; CSTF3 contributes to platinum resistance in ovarian cancer through alternative polyadenylation of lncRNA NEAT1 and generating the short isoform NEAT1_1; Authors - Xin Luo